Journal: bioRxiv
Article Title: SenCat: Cataloging human cell senescence through multiomic profiling of multiple senescent primary cell types
doi: 10.64898/2026.02.05.703986
Figure Lengend Snippet: (A) Schematic of the experimental design for induction of systemic senescence with doxorubicin and sample collection across time points. (B) Cluster identities assigned to doxorubicin-treated lung samples profiled by snRNA-seq. (C) UMAP projection of lung samples colored by cluster identity. (D) UMAP projections of senescent cells in lung samples across time points. (E) Bar plot showing the number of senescent cells from lung per cluster and condition. (F,G) GSEA plots of gene set association scores for p53, EMT, NF-κB, Apoptosis, and Hypoxia hallmark pathways (F) and senescence signature lists SenePy, SeneSig, SenMayo, hUSI (G); in fibroblast, epithelial, and endothelial clusters on Day 6. (H) Schematic of the analysis pipeline applied to published aging lung snRNA-seq datasets. (I) UMAP projection of aging lung samples showing senescent cell distribution by age group. (J) Schematic of ligand-receptor inference analysis between senescent fibroblasts and non-senescent epithelial cells. (K) Chord diagram displaying ligand-receptor interactions between senescent fibroblasts (sender cells) and non-senescent epithelial cells (receiver cells) inferred through CellPhoneDB. (L) Dot plot showing expression of the specified ligands across fibroblast clusters at 23 months of age. (M) Schematic and GSEA plot explaining and displaying TGFβ signaling pathway increase during aging in non-senescent epithelial cells at 23 months versus 3 months. See also Figures S11-13 .
Article Snippet: HSAEC lung epithelial cells (ATCC, PCS-301-010) were cultured using an Airway Epithelial Cell Basal Medium plus Bronchial Epithelial Cell Growth Kit (ATCC).
Techniques: Expressing